Associate Professor, Immunology
E1056 Biomedical Science Tower
Pittsburgh, PA 15261
BS (Biology), Boston College, 1988.
PhD (Immunology), Univ. of Calif., San Diego, 1996.
Post-Doc, University of California, San Francisco, 1996-2000.
Adjunct Faculty, University of California, San Francisco 2000-2003
My lab is currently pursuing several projects:
1. The role of TIM family molecules in T cell activation and differentiation
This project currently involves the study of TIM-1, a novel protein of the T cell immunoglobulin and mucin domain family. We have found that TIM-1 can provide a co-stimulatory signal, along with the TCR, that affects T cell activation and differentiation. We are trying to determine what signaling pathways are activated by TIM-1 as well as performing structure/function studies to determine how TIM-1 couples to these pathways.
2. Activation of NF-kB by the Akt kinase
We found that Akt can induce NF-kB-dependent transcription, in cooperation with signals from PKC. We are currently working to identify direct targets of Akt in this pathway, as well as its consequences for T cell activation and CD28 co-stimulation. One target that we have already identified is a MAP3K family member called Cot, or Tpl-2. We have found that Akt can phosphorylate Cot and modulate its function. We are currently trying to determine how phosphorylation of Cot affects its function.
Narayan P, Holt B, Tosti R and Kane LP. (2006) CARMA1 is required for Akt-mediated NF-KB activation in T cells. Mol Cell Biol. 26:2327.
Knickelbein J, de Souza AJ, Narayan P, Tosti R and Kane LP. (2006) Cutting Edge: Inhibition of T cell activation by TIM-2. J. Immunol. 177:4966.