Courtney Andersen studies the role of estrogen receptor-alpha in ovarian cancer.
Chris Barnes investigates the structural details by which transcription factor activity regulates RNA Polymerase II during the universal process of eukaryotic gene expression.
Soma Jobaggy studies nitrated fatty acid pharmacology and the antioxidant response in hypertensive end-organ damage.
Allison Nagle studies growth factor receptor signaling in breast cancer.
Shivendra Singh, PhD
Professor and UPMC Chair in Cancer Prevention Research
2.32a Hillman Cancer Center
5117 Centre Avenue, Pittsburgh, Pa 15232

Email:
svs2@pitt.edu
Phone: 412-623-3262

Fax: 412-623-7828


Education

MSc (Biochemistry), Banaras Hindu University, India, 1979
PhD (Biochemistry), Banaras Hindu University, India, 1984
Postdoctoral Fellow, University of Texas Medical Branch at Galveston, 1984-1988



Research Areas
Cancer Pharmacology
Drug Discovery
Signal Transduction
Photo of Shivendra Singh, PhD

Cancer chemoprevention is a relatively new but rapidly emerging sub-discipline in oncology and signifies the use of natural or synthetic agents to reverse or delay the process of carcinogenesis. Long latency of most epithelial cancers presents a large window of opportunity for intervention to prevent or slow disease progression. The research interests of the Singh laboratory include molecular characterization of novel cancer chemopreventive agents and rational design of mechanism-driven combination chemoprevention regimens. Cellular and transgenic animal models are used to screen potential cancer chemopreventive constituents from dietary and medicinal plants. Cutting edge cellular, molecular biological, Omics (metabolomics and proteomics), structural biology, and imaging techniques (MRI and bioluminescence) are used to (a) determine the mechanism of action of promising cancer chemopreventive agents, (b) monitor effects on cancer progression, and

(c) identify biomarkers predictive of tissue exposure and possibly response. Some of the agents under active investigation in the Singh laboratory include: cruciferous vegetable-derived isothiocyanates, garlic-derived organosulides, and medicinal plant constituent withaferin A. As an example, recent published work from the Singh laboratory indicates suppression of glycolysis in mammary cancer prevention by withaferin A in a transgenic mouse model (JNCI, In Press, 2013). Likewise, complementary cellular and molecular biological, targeted proteomics, and molecular modeling techniques were used to identify beta-tubulin as a novel target of cancer cell growth arrest by withaferin A (WA).





Important Publications
Hahm ER, J Lee, SH Kim, A Sehrawat, JA Arlotti, SS Shiva, R Bhargava and SV Singh.  Metabolic alterations in mammary cancer prevention by withaferin A in a clinically-relevant mouse model.  J Natl Cancer Inst, in press, 2013.
Singh SV, SH Kim, A Sehrawat, JA Arlotti, ER Hahm, K Sakao, JH Beumer, RC Jankowitz, K Chandra-Kuntal, J Lee, AA Powolny and R Dhir.  Biomarkers of phenethyl isothiocyanate-mediated mammary cancer chemoprevention in a clinically relevant mouse model.  J Natl Cancer Inst 104:1228-1239, 2012.
Powolny AA, A Bommareddy, ER Hahm, DP Normolle, JH Beumer, JB Nelson and SV Singh.  Chemopreventative potential of the cruciferous vegetable constituent phenethyl isothiocyanate in a mouse model of prostate cancer.  J Natl Cancer Ins 103:571-584, 2011.
Xiao D and SV Singh.  p66shc is indispensable for phenethyl isothiocyanate-induced apoptosis in human prostate cancer cells.  Cancer Res 70:3150-3158, 2010.
Bommareddy A, ER Hahm, D Xiao, AA Powolny, AL Fisher, Y Jiang and SV Singh.  Atg5 regulates phenethyl isothiocyanate-induced autophagic and apoptotic cell death in human prostate cancer cells.  Cancer Res 69:3704-3712, 2009.

STUDENT NEWS


UPCOMING EVENTS
11/27/2017 8:30 AM Molecular Pharmacology Journal Club
Lloyd Harvey


11/30/2017 12:00 PM Pharmacology & Chemical Biology Seminar Series
Jeffrey L. Brodsky, Ph.D.


12/4/2017 8:30 AM Molecular Pharmacology Journal Club
Andrew Lamade


Pharmacology and Chemical Biology Event Calendar

Program Achievements

Molecular Pharmacology Graduate Program Ranked #2 in National Research Council Rankings

Molecular Pharmacology Graduate Program Ranked #2 in Faculty Scholarly Productivity Index


Outcomes:  Time to disseration, last five graduating clasess:  4.5 years, Completion Rate: 84.8%

Ranked #12 in National of Institute of Health funding of departments of Pharmacology

Ranked in the top 15 in funding for twenty consecutive years




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