Department of Pharmacology & Chemical Biology at the University of Pittsburgh
Friedman Lab W1303 Thomas E. Starzl Biomedical Tower
200 Lothrop Street, Pittsburgh, PA 15213

Friedman, Romero and Vilardaga labs 

Studies in Dr. Friedman's laboratory focus primarily on the mechanism and regulation of calcium transport across cell membranes. Experimental work is presently directed toward characterizing the interactions between the calcium-sensing receptor (CaSR) and parathyroid hormone (PTH) on calcium absorption by renal thick ascending limbs and distal tubule cells.

Problems under investigation include: 1) delineating the role of the CaSR in controlling basal and PTH-dependent calcium absorption; 2) determining the role of calcium channel subunits in regulating distal tubule calcium absorption; and, 3) defining structural elements in the PTH receptor responsible for signaling and internalization.

Techniques applied to these studies include cell culture models, isolated perfused single tubules, and clearance studies in transgenic mice. Single cell fluorescence, tracer flux measurements, heterologous receptor expression, antisense depletion techniques, biochemical and molecular analyses of second messenger formation are routinely applied. Elucidation of the mechanism of PTH receptor signaling will not only explain the physiological action of PTH at one of its principal target sites but also provide insight into novel mechanisms by which cell specific signaling is conferred. The resulting information will be valuable in understanding mineral ion homeostasis under normal conditions, as well as disordered calcium balance in renal failure, hyperparathyroidism, or osteoporosis. The results will provide insights into analogs that may be candidates for selective therapeutic applications.

Peter A. Friedman, PhD

Tatyana Mamonova, PhD

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