Department of Pharmacology & Chemical Biology at the University of Pittsburgh
Dario Vitturi, PhD
Research Assistant Professor
E1341B Biomedical Science Tower
Pittsburgh, PA 15261

Email:
dav28@pitt.edu
Phone: 412-648-8160

Fax: 412-648-2229


Education

B.S. (Biochemistry), University of the Republic, Uruguay

Ph.D. (Molecular and Cellular Pathology), University of Alabama at Birmingham



Research Areas
Redox Pharmacology
Pharmacology of Cell and Organ Systems
Signal Transduction
Photo of Dario Vitturi, PhD

Dr. Vitturi’s research focuses on the mechanisms by which biological systems harness the generation of free radicals and other reactive species to modulate cell signaling and maintain physiological homeostasis. Dr. Vitturi has been involved with the study of nitrite as a physiologically relevant precursor for nitric oxide (NO) formation that becomes activated under conditions of hypoxia and/or metabolic acidosis via reaction with metalloproteins. Nitrite has been shown to be a promising therapeutic intervention for many conditions associated with uncontrolled inflammation, decreased NO availability and ischemia-reperfusion events. Importantly, NO generation is just one aspect of the biological reactivity of nitrite, with this molecule also acting as a precursor for the formation of secondary species such as nitrated fatty acids, S-nitrosothiols and N-nitrosamines, all of which are capable of mediating specific cellular responses. In this regard, Dr. Vitturi’s current research interests encompass the study of biochemical pathways leading to the formation of these secondary species, the elucidation of their allied biological actions and ultimately the development of clinically viable interventions for their modulation under pathological conditions.




Important Publications
Vitturi DA, CS Chen, SR Woodcock, SR Salvatore, G Bonacci, JR Koenitzer, NA Stewart, N Wakabayashi, TW Kensler, BA Freeman and FJ Schopfer.  Modulation of nitro-fatty acid signaling:  Prostaglandin reductase-1 is a nitroalkene reductase. J Biol Chem 288:25626-25637, 2013.
Salvatore SR, DA Vitturi, PR Baker, G Bonacci, JR Koenitzer, SR Woodcock, BA Freeman and FJ Schopfer.  Characterization and quantification of endogenous fatty acid nitroalkene metabolites in human urine.  J Lipid Res 54:1998-2009, 2013.
Vitturi DA, CW Sun, VM Harper, B Thrash-Williams, N Cantu-Medellin, BK Chacko, N Peng, Y Dai, JM Wyss, T Townes and RP Patel.  Antioxidant functions for the hemoglobin ß93 cysteine residue in erythrocytes and in the vascular compartment in vivo.  Free Radic Biol Med 55:119-129, 2013.
Rodriguez C, DA Vitturi, J He, M Vandromme, A Brandon, A Hutchings, LW Rue, JD Kerby and RP Patel.  Sodium nitrite therapy attenuates the hypertensive effects of HBOC-201 via nitrite reduction.  Biochem J 422:423-432, 2009.
Vitturi DA, X Teng, JC Toledo, S Matalon, JR Lancaster and RP Patel.  Regulation of nitrite transport in red blood cells by hemoglobin oxygen fractional saturation.  Am J Physiol Heart Circ Physiol 296:H1394-H1407, 2009.
Isbell TS, CW Sun, LC Wu, X Teng, DA Vitturi, BG Branch, CG Kevil, N Peng, JM Wyss, N Ambalavanan, L Schwiebert, J Ren, KM Pawlik, MB Renfrow, RP Patel and TM Townes.  SNO-hemoglobin is not essential for red blood cell-dependent hypoxic vasodilation.  Nat Med 14:773-777, 2008.
Möller MN, Q Li, DA Vitturi, JM Robinson, JR Lancaster and A Denicola.  Membrane "lens" effect:  Focusing the formation of reactive nitrogen oxides from the *NO/02 reaction.  Chem Res Toxicol 20:709-714, 2007.




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