Department of Pharmacology & Chemical Biology at the University of Pittsburgh
Eugenia Cifuentes-Pagano, PhD
Research Instructor
E1228 Thomas E. Starzl Biomedical Science Tower
200 Lothrop Street, Pittsburgh, PA 15213

Email:
mec110@pitt.edu
Phone: 412-648-2610


Education

BS (Biology), University of Chile, 1984
MS (Cell Biology), University of Chile, 1984
PhD (Physiology and Biophysics), State University of New York at Stony Brook, 1994



Research Areas
Redox Pharmacology
Pharmacology of Cell and Organ Systems
Drug Discovery
Photo of Eugenia Cifuentes-Pagano, PhD

Dr. Cifuentes-Pagano’s research interests focus on the understanding of the molecular mechanisms of action of novel NADPH oxidase isoforms and their regulation in the vasculature. The phagocyte NADPH oxidase (or respiratory burst oxidase) is a well-characterized reactive oxygen species (ROS)-generating system that catalyzes the one-electron reduction of oxygen to O2-, the precursor to a variety of other reactive oxygen species. The NADPH oxidase paradigm is a multi-subunit enzyme complex that includes two membrane-spanning subunits, p22-phox and nox2 , and three cytoplasmic subunits, p40-phox, p47-phox and p67-phox. Our laboratory was the first to discover a nox2-based oxidase in the vasculature and to develop specific inhibitors targeting this robust source of ROS. Since that initial discovery, various isoforms of NADPH oxidase have been described which differ from the nox2 system in unique modifications of their nox-subunit amino acid sequence as well as the cytoplasmic components that they require. Besides their structural differences, the various isoforms present differential tissue and cellular distribution. The multi-level complexity of this family of proteins provides an opportunity to develop new tools to dissect the role of each of the isoforms in vascular function and pathology.

http://paganolab.pitt.edu/





Important Publications
Csanyi G, E Cifuentes-Pagano, I Al Ghouleh, DJ Ranayhossaini, L Egana, LR Lopes, HM Jackson, EE Kelley and PJ Pagano.  Nox2 B-loop peptide, Nox2ds, specifically inhibits the NADPH oxidase Nox2.  Free Radic Biol Med 51:1116-1125, 2011.
Ardanaz N, XP Yang, ME Cifuentes, MJ Haurani, KW Jackson, TD Liao, OA Carretero and PJ Pagano.  Lack of glutathione peroxidase 1 accelerates cardiac-specific hypertrophy and dysfunction in angiotensin II hypertension.  Hypertension 55:116-123, 2010.
Haurani MJ, ME Cifuentes, AD Shepard and PJ Pagano. Nox4 oxidase overexpression specifically decreases endogenous Nox4 mRNA and inhibits angiotensin II-induced adventitial myofibroblast migration. Hypertension 52(1):143-149, 2008.
Cifuentes ME and PJ Pagano. Targeting reactive oxygen species in hypertension. Curr Opin Nephrol Hypertens 15:179-186, 2006.
Cifuentes ME and PJ Pagano. c-Src and smooth muscle NAD(P)H oxidase: Assembling a path to hypertrophy. Arterioscler Thromb Vasc Biol 23:919-921, 2003.
Rey FE, ME Cifuentes, A Kiarash, MT Quinn and PJ Pagano. Novel competitive inhibitor of NAD(P)H oxidase assembly attenuates vascular O2- and systolic blood pressure in mice. Circ Res 89:408-414, 2001.
Cifuentes ME, F Rey, OA Carretero and PJ Pagano. Upregulation of p67(phox) and gp91(phox) in aortas from angiotensin II-infused mice. Am J Physiol Heart Circ Physiol 279:H2234-H2240, 2000.
Pagano PJ, JK Clark, ME Cifuentes-Pagano, SM Clark, GM Callis and MT Quinn. Localization of a constitutively active, phagocyte-like NADPH oxidase in rabbit aortic adventitia: enhancement by angiotensin II. Proc Natl Acad Sci USA 94:4483-14488, 1997.




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