The basic investigation and clinical research activities of the Freeman Laboratory focus on the eukaryotic cell production, reactions and signal transduction properties of oxidizing and free radical inflammatory mediators (e.g., superoxide, hydrogen peroxide, nitric oxide (NO), peroxynitrite, nitrogen dioxide, oxidized/nitrated lipids).  In particular, we are interested in the actions of these species as both redox signaling mediators, as pathogenic agents in inflammatory diseases and in the case of nitro-fatty acids as drug candidates.  Our observations regarding O₂ and NO-derived reactive species have lent new insight into mechanisms of redox-dependent cell signaling, post-translational protein modification and have revealed new therapeutic strategies for treating acute inflammation, metabolic syndrome, respiratory disorders and cardiovascular diseases.