Romero Lab

The Romero laboratory focuses on the study of the regulation of G protein coupled receptors (GPCR) by intracellular scaffold proteins. We focus primarily on the function and traffic of the parathyroid hormone receptor (PTHR) and the Frizzled family of receptors and their regulation by PDZ proteins.

Problems under investigation include: 1) the modulation of receptor traffic and function by PDZ scaffolds; 2) the role of PDZ proteins in the regulation of the Wnt/Frizzled signaling pathways; and 3) the role of specific PDZ scaffolds in tumor biology.

We apply multiple techniques to these studies, ranging from hard-core biophysical and imaging approaches to studies in whole animals and human patient samples. For example, we apply single-cell and single-molecule fluorescence techniques to the study of the dynamics and traffic of receptors on the plasma membrane. We also use fluorescence correlation spectroscopy approaches to study protein-protein interactions in live cells, confocal and electron microscopy to study the distribution of specific protein targets in subcellular compartments, and other rigorous, quantitative imaging approaches. This work is complemented with protein chemistry, membrane biochemistry and molecular biology studies in normal and transformed cells, and testing of specific signaling models in transgenic animals. The laboratory collaborates seamlessly with the Friedman and Vilardaga labs, sharing equipment, reagents, and research resources.

Guillermo G. Romero, PhD
Associate Professor

Guillermo G. Romero, PhD

Journal Articles

Wheeler DS, SR Barrick, MJ Grubisha, AM Brufsky, PA Friedman and G Romero.  Direct interaction between NHERF1 and frizzled regulates beta-catenin signaling.  Oncogene 30:32-42, 2011.
G Romero, WB Sneddon, Y Yang, D Wheeler, HC Blair and PA Friedman.  Parathyroid hormone receptor directly interacts with dishevelled to regulate beta-catenin signaling and osteoclastogenesis.  J Biol Chem 285:14756-14763, 2010.
Kraft CA, JL Garrido, L Leiva-Vega and G Romero.  Quantitative analysis of protein-lipid interactions using tryptophan fluorescence.  Science Signaling 2:p14, 2009.
Garrido JL, D Wheeler, L Leiva-Vega, P Friedman and G Romero.  Role of phospholipase D in parathyroid hormone type 1 receptor trafficking and signaling.  Molecular Endocrinology 23:2048-2059, 2009.
Wheeler D, JL Garrido, A Bisello, YK Kim, PA Friedman and G Romero G. Regulation of PTH1R dynamics, traffic, and signaling by the Na+/H+ exchanger regulatory factor-1 (NHERF1) in rat osteosarcoma ROS 17/2.8 cells. Mol Endocrinol 22(5):1163-1170, 2008.
Kraft CA, JL Garrido, L Leiva-Vega, E Fluharty and G Romero.  Role of phosphatidic acid in the coupling of the extracellular-signal regulated kinase (ERK) cascade.  J Biol Chem 283:33636-33645, 2008.