Schopfer Lab

Tha Schopfer Lab studies the role of bioactive fatty acids in physiology and pathology. Using cutting edge mass spectrometry-based lipidomic approaches we identified novel signaling lipids in vivo. Using cellular and animal models we investigate the biological and biochemical activity of these compounds. Among the fatty acids we discovered, we focus our investigation on nitrated and furan fatty acids. Nitrated fatty acids are potent anti-inflammatory and anti-oxidant endogenous fatty acids that we validated in preclinical animal models successfully moved to Phase I and II clinical trials. Furan fatty acids are an important and neglected component of fish oils and we investigate their signaling activities. Overall, to further support our early discovery efforts and pharmacological developments, we use organic synthesis, isotopically labeled standards, pharmacokinetic evaluations, formulation development and pharmacodynamics approaches. 

Soma Jobbagy, PhD
MSTP Postdoctoral Associate


Sonia Salvatore
Research III


Francisco J. Schopfer, PhD
Associate Professor & Vice Chair for Biotechnology Development

Francisco J. Schopfer, PhD

Journal Articles

Jobbagy S, DA Vitturi, SR Salvatore, L Turell, MF Pires, E Kansanen, C Batthyany, JR Lancaster, Jr., BA Freeman and FJ Schopfer.  Electrophiles modulate glutathione reductase activity via alkylation and upregulation of glutathione biosynthesis. Redox Biol 21, 101050, 2019.
Fazzari M, DA Vitturi, SR Woodcock, SR Salvatore, BA Freeman and FJ Schopfer. Electrophilic fatty acid nitroalkenes are systemically transported and distributed upon esterification to complex lipids. J Lipid Res 60:388-399, 2019.
Hansen AL, GJ Buchan, M Ruhl, K Mukai, SR Salvatore, E Ogawa, SD Andersen, MB Iversen, AL Thielke, C Gunderstofte, M Motwani, CT Moller, AS Jakobsen, KA Fitzgerald, J Roos, R Lin, TJ Maier, R Goldbach-Mansky, CA Miner, W Qian, JJ Miner, RE Rigby, J Rehwinkel, MR Jakobsen, H Arai, Y Taguchi, FJ Schopfer, D Olagnier and CK Holm. Nitro-fatty acids are formed in response to virus infection and are potent inhibitors of STING palmitoylation and signaling. Proc Natl Acad Sci U S A 115:E7768-E7775, 2018,
Prentice KJ, SG Wendell, Y Liu, JA Eversley, SR Salvatore, H Mohan, SL Brandt, AC Adams, X Serena Wang, D Wei, GA FitzGerald, TB Durham, CD Hammond, KW Sloop, C Skarke, FJ Schopfer and MB Wheeler.  CMPF, a metabolite formed upon prescription omega-3-acid ethyl ester supplementation, prevents and reverses steatosis. EBioMedicine 27:200-213, 2018,
Turell L, DA Vitturi, EL Coitino, L Lebrato, MN Moller, C Sagasti, SR Salvatore, SR Woodcock, B Alvarez and FJ Schopfer. The chemical basis of thiol addition to nitro-conjugated linoleic acid, a protective cell-signaling lipid. J Biol Chem 292:1145-1159, 2017.
Fazzari M, N Khoo, SR Woodcock, L Li, BA Freeman and FJ Schopfer.Generation and esterification of electrophilic fatty acid nitroalkenes in triacylglycerides. Free Radic Biol Med 87:113-124, 2015.
Vitturi DA, L Minarrieta, SR Salvatore, EM Postlethwait, M Fazzari, G Ferrer-Sueta, JR Lancaster, Jr., BA Freeman and FJ Schopfer. Convergence of biological nitration and nitrosation via symmetrical nitrous anhydride. Nat Chem Biol 11:504-510, 2015.
Vitturi DA, CS Chen, SR Woodcock, SR Salvatore, G Bonacci, JR Koenitzer, NA Stewart, N Wakabayashi, TW Kensler, BA Freeman and FJ Schopfer. Modulation of nitro-fatty acid signaling: prostaglandin reductase-1 is a nitroalkene reductase. J Biol Chem 288:25626-25637, 2013.
Bonacci G, PR Baker, SR Salvatore, D Shores, NK Khoo, JR Koenitzer, DA Vitturi, SR Woodcock, F Golin-Bisello, MP Cole, S Watkins, C St Croix, CI Batthyany, BA Freeman and FJ Schopfer. Conjugated linoleic acid is a preferential substrate for fatty acid nitration. J Biol Chem 287:44071-44082, 2012.
Groeger AL, C Cipollina, MP Cole, SR Woodcock, G Bonacci, TK Rudolph, V Rudolph, BA Freeman and FJ Schopfer.  Cyclooxygenase-2 generates anti-inflammatory mediators from omega-3 fatty acids.  Nat Chem Biol 6:433-441, 2010.

Reviews

Hansen AL, K Mukai, FJ Schopfer, T Taguchi and CK Holm. STING palmitoylation as a therapeutic target. Cell Mol Immunol 16:236-241, 2019.
Schopfer FJ and NKH Khoo. Nitro-fatty acid logistics: Formation, biodistribution, signaling, and pharmacology. Trends Endocrinol Metab 30:505-519, 2019.
Schopfer FJ, DA Vitturi, DK Jorkasky and BA Freeman. Nitro-fatty acids: New drug candidates for chronic inflammatory and fibrotic diseases. Nitric Oxide 79:31-37, 2018,
Freeman BA, VB O'Donnell and FJ Schopfer. The discovery of nitro-fatty acids as products of metabolic and inflammatory reactions and mediators of adaptive cell signaling. Nitric Oxide 77:106-111, 2018.
Schopfer FJ, C Cipollina and BA Freeman. Formation and signaling actions of electrophilic lipids. Chem Rev 111:5997-6021, 2011.