Vilardaga Lab

The Vilardaga laboratory carries an interdisciplinary research program aimed at elucidating molecular mechanisms of signal transduction mediated by G-protein coupled receptors in renal, skeletal and other cell systems.

Lisa Clark
Predoctoral Fellow

Fei Fang, PhD
Postdoctoral Associate

Jean-Pierre Vilardaga, PhD

Alex White, PhD
Postdoctoral Associate

Alex White

Kunhong (Kevin) Xiao, MD, PhD
Research Associate Professor

Jean-Pierre Vilardaga, PhD

Journal Articles

Chelocha RW, SH Gellman, JP Vilardaga and TJ Gardella. Signal transduction at type -1  parathyroid hormone receptor: Mechanistic studies provide insight into GPCR signaling and highlight therapeutic opportunities. Nature Review Endocrinology 11: 712-724, 2015. 
Gardella TJ and JP Vilardaga.  The parathyroid hormone receptors: Family B GPCRs.  Pharmacol Reviews 67: 310-337, 2015.
Vilardaga JP, FG Jean-Alphonse and TJ Gardella. Endosomal generation of cAMP in GPCR signaling. Nature Chemical Biology 10: 700-706, 2014.
Gidon A, MM Al-Bataineh, FG Jean-Alphonse, H Stevenson, T Watanabe, C Louet, A Khatri, G Calero, NM Pastor-Soler, TJ Gardella and JP Vilardaga. Endosomal GPCR signaling turned off by negative feedback actions of PKA and v-ATPase. Nature Chem Biol 10: 707-711, 2014.
Wehbi VL, H Stevenson, TN Feinstein, G Calero, G Romero and JP Vilardaga.  Non-canonical GPCR signaling arising from a PTH receptor-arrestin-Gßy complex.  Proc Natl Acad Sci USA 110:1530-1535, 2013.
Feinstein TN, N Yui, MJ Webber, VL Wehbi, HP Stevenson, J Darwin King, Jr., KR Hallows, D Brown, R Bouley and JP Vilardaga.  Noncanonical control of vasopressin receptor type 2 signaling by retromer and arrestin. J. Biol. Chem 288:27849-27860, 2013.
Vilardaga JP, TJ Gardella, VL Wehbi and TN Feinstein.  Non-canonical signaling of the PTH receptor.  Trends Pharmacol Sci 33:423-431, 2012.
Hanyu R, VL Wehbi, T Hayata, S Moriya, TN Feinstein, Y Yoichi Ezura, M Nagao, Y Saita, H Hemmi, T Notomi, T Nakamoto, E Schipani, S Takeda, K Kaneko, H Kurosawa, G Karsenty, KM Kronenberg, JP Vilardaga and M Noda.  Anabolic action of PTH regulated by the ß2-adrenergic receptor.  Proc Natl Acad Sci USA 109:7433-7438, 2012.
Feinstein TN, VL Wehbi, JA Ardura, DA Wheeler, S Ferrandon, TJ Gardella and JP Vilardaga.  Retromer terminates the generation of cAMP by internalized PTH-receptors.  Nature Chemical Biology 7:278-284,2011.
Ferrandon S, TN Feinstein, C Castro, B Wang R Bouley, JT Potts, TJ Gardella and JP Vilardaga.  Sustained cyclic AMP production by parathyroid hormone receptor endocytosis.  Nature Chemical Biology 5:734-742, 2009.
Okazaki M, S Ferrandon, JP Vilardaga, ML Bouxsein, JT Potts and TJ Gardella. Prolonged signaling at the parathyroid hormone receptor by peptide ligands targeted to a specific receptor conformation. Proc. Natl. Acad. Sci. USA 105:16525-16530, 2008.
Vilardaga JP, OV Nikolaev, K Lorenz, S Ferrandon, Z Zhuang and MR Lohse. Conformational cross-talk between a2A-adrenergic and m-opioid receptors control cell signaling. Nature Chemical Biology 4:126-131, 2008.
Rochais F, JP Vilardaga, VO Nikolaev, M Bünemann, MJ Lohse and S Engelhardt. Real-time optical recording of b1-adrenergic receptor activation reveals supersensitivity of the Arg389 variant to carvedilol. J. Clin. Invest. 117:229-235, 2007.
Vilardaga JP, M  Bünemann, C Krasel, M Castro and MJ Lohse.  Measurement of the millisecond activation switch of G protein-coupled receptors in living cells.  Nature Biotechnology 21:807-812, 2003.

Alex White

Journal Articles

Dube, S.; Dube, H.; Green, N.B.; Larsen, E.M.; White, A.; et al. In vivo delivery and activation of masked fluorogenic hydrolase substrates by endogenous hydrolases in C. elegans. ChemBioChem. 2017 Sep 19; 18(18): 1807-1813. doi: 10.1002/cbic.201700278. Epub 2017 Aug 7.PMCID:PMC5605452

Kunhong (Kevin) Xiao, MD, PhD

Journal Articles

Brady DC, Crowe MS, Turski ML, Hobbs GA, Yao XJ, Apirat C, Knapp S, Xiao K, Campbell SL, Thiele DJ and Counter CM. Copper is required for oncogenic BRAF signaling and tumorigenesis. Nature 509:492-496, 2014.
Shukla A, Westfield G, Xiao K, Reis RI, Huang LY, Tripathi-Shukla P, Qian J, Li S, Blanc A, Oleskie AN, Dosey AM, Su M, Liang CR, Gu LL, Shan JM, Chen X, Hanna R, Choi M, Yao XJ, Klink BU, Kahsai AW, Sidhu S, Koide S, Penczek PA, Kossiakoff AA, Woods V, Kobilka BK, Skiniotis G and Lefkowitz RJ. Visualization of arrestin recruitment by a G- protein-coupled receptor. Nature 512:218-22, 2014.

Kahsai AW, Rajagopal S, Sun J and Xiao K.  Monitoring protein conformational changes and dynamics using stable-isotope labeling and mass spectrometry. Nature Protocols 9:1301–1319, 2014.
Shukla A, Manglik A, Kruse A, Xiao K, Reis A, Tseng W, Staus D, Hilger D, Uysal S, Huang L, Paduch M, Tripathi-Shukla P, Koide A, Koide S, Weis W, Kossiakoff A, Kobilka B and Lefkowitz RJ. Structure of active β-arrestin1 bound to a G protein-coupled receptor phosphopeptide. Nature 497:137-141, 2013.
Kahsai AW, Xiao K, Rajagopal S, Ahn S, Shukla AK, Sun J, Oas TG and Lefkowitz RJ.   Multiple ligand-specific conformations of the β2-adrenergic receptor. Nature Chemical Biology 7:692-700, 2011.
Nobles KN, Xiao K, Ahn S, Shukla AK, Lam CL, Rajagopal S, Bressler EA, Hara RM, Shenoy SK, Gygi SP and Lefkowitz RJ.   Distinct GRK phosphorylation sites on the β2AR: A “bar code” which differentially encodes β-arrestin functions. Science Signaling 4:ra51, 2011.
Xiao K and Shenoy SK. β2AR lysosomal trafficking is regulated by ubiquitination of lysyl residues in two distinct receptor domains. Journal of Biological Chemistry 286: 12785-12795, 2011.