Schopfer Lab

Tha Schopfer Lab studies the role of bioactive fatty acids in physiology and pathology. Using cutting edge mass spectrometry-based lipidomic approaches we identified novel signaling lipids in vivo. Using cellular and animal models we investigate the biological and biochemical activity of these compounds. Among the fatty acids we discovered, we focus our investigation on nitrated and furan fatty acids. Nitrated fatty acids are potent anti-inflammatory and anti-oxidant endogenous fatty acids that we validated in preclinical animal models successfully moved to Phase I and II clinical trials. Furan fatty acids are an important and neglected component of fish oils and we investigate their signaling activities. Overall, to further support our early discovery efforts and pharmacological developments, we use organic synthesis, isotopically labeled standards, pharmacokinetic evaluations, formulation development and pharmacodynamics approaches. 

Sonia Salvatore
Research III

Francisco J. Schopfer, PhD
Associate Professor & Vice Chair for Biotechnology Development

Francisco J. Schopfer, PhD

Bonacci G, EK Asciutto, SR Woodcock, SR Salvatore, BA Freeman and FJ Schopfer.  Gas-phase fragmentation analysis of nitro-fatty acids.  J Am Soc Mass Spectr 22:1534-1551, 2011.
Schopfer FJ, C Cipollina and BA Freeman.  Formation and signaling actions of electrophilic lipids.  Chem Rev 111:5997-6021, 2011.
Kansanen E, G Bonacci, FJ Schopfer, SM Kuosmanen, KI Tong, H Leinonen, SR Woodcock, M Yamamoto, C Carlberg, S Yla-Herttuala, BA Freeman and AL Levonen.  Electrophilic nitro-fatty acids activate NRF2 by a KEAP1 cysteine 151-independent mechanism.  J Biol Chem 286:14019-14027, 2011.
Bonacci G, FJ Schopfer, CI Batthyany, TK Rudolph, V Rudolph, NK Khoo, EE Kelley and BA Freeman.  Electrophilic fatty acids regulate matrix metalloproteinase activity and expression.  J Biol Chem 286:16074-16081, 2011.
Schopfer FJ, MP Cole, AL Groeger, CS Chen, NK Khoo, SR Woodcock, F Golin-Bisello, UN Motanya, Y Li, J. Zhang, MT Garcia-Barrio, TK Rudolph, V Rudolph, G Bonacci, PR Baker, HE Xu, C Batthyany, YE Chen, TM Hallis and BA Freeman.  Covalent peroxisome proliferator-activated receptor gamma adduction by nitro-fatty acids:  Selective ligand activity and anti-diabetic signaling actions.  J Biol Chem 285:12321-12333, 2010.
Groeger AL, C Cipollina, MP Cole, SR Woodcock, G Bonacci, TK Rudolph, V Rudolph, BA Freeman and FJ Schopfer.  Cyclooxygenase-2 generates anti-inflammatory mediators from omega-3 fatty acids.  Nat Chem Biol 6:433-441, 2010.
Rudolph V, TK Rudolph, FJ Schopfer, G Bonacci, SR Woodcock, MP Cole, PR Baker, R Ramani and BA Freeman.  Endogenous generation and protective effects of nitro-fatty acids in a murine model of focal cardiac ischaemia and reperfusion.  Cardiovasc Res 85:155-166, 2010.
Zhang J, L Villacorta, L Chang, Z Fan, M Hamblin, T Zhu, CS Chen, MP Cole, FJ Schopfer, CX Deng, MT Garcia-Barrio, YH Feng, BA Freeman and YE Chen.  Nitro-oleic acid inhibits angiotension II-induced  hypertension.  Circ Res 107:540-548, 2010.