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Primary Faculty
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Jonathan Pacheco Romero, PhD
Jonathan Pacheco Romero, PhD
Research Instructor
W1301 Thomas E. Starzl Biomedical Science Tower
203 Lothrop Street
Pittsburgh, PA 15261
jep160@pitt.edu
Phone: 412-608-4863
Education
BS (Biology), Michoacan University of San Nicolás de Hidalgo (UMSNH), 2010
PhD (Biomedical Sciences), National Autonomous University of Mexico (UNAM), 2016
Research Details
Publications
Research Areas
Hormone Signaling and Action
Receptor Pharmacology
Dr. Pacheco's research focuses on the dynamic regulation of G protein-coupled receptors (GPCRs), particularly delving into the intricate workings of the parathyroid hormone receptor (PTHR). He employs single-molecule visualization techniques and live cell imaging to explore the spatial and temporal organization of PTHR and its regulatory proteins, aiming to unravel the complexities of G-protein mediated signaling pathways. Furthermore, Dr. Pacheco utilizes advanced high-throughput methods, such as Förster resonance energy transfer (FRET) and bioluminescence resonance energy transfer (BRET), to understand the molecular structure and cellular interactions involved in ligand-receptor binding within the PTHR. His comprehensive research endeavors seek to shed light on PTHR functionality and its implications in comprehending and manipulating cellular signaling pathways.
Journal Articles
Wills RC, Doyle CP, Zewe JP,
Pacheco J
, Hansen SD and Hammond GRV. A novel homeostatic mechanism tunes PI(4,5)P2-dependent signaling at the plasma membrane. J Cell Sci, 2023.
Pacheco J
, Cassidy AC, Zewe JP, Wills RC and Hammond GRV. PI(4,5)P2 diffuses freely in the plasma membrane even within high-density effector protein complexes. Journal of Cell Biology 222, 2023.
Walpole, GFW,
Pacheco J
, Chauhan N, Clark J, Anderson KE, Abbas YM, Brabant-Kirwan D, Montan~o-Rendo´n F, Liu Z, Zhu H, et al. Kinase-independent synthesis of 3- phosphorylated phosphoinositides by a phosphotransferase. Nat Cell Biol 24, 2022.
Goulden BD,
Pacheco J
, Dull A, Zewe JP, Deiters A and Hammond GRV. A high-avidity biosensor reveals plasma membrane PI(3,4)P 2 is predominantly a class I PI3K signaling product. Journal of Cell Biology, 2019.
Pacheco J
, Dominguez L, Bohorquez-Hernandez A, Asanov A and Vaca L. A cholesterol-binding domain in STIM1 modulates STIM1-Orai1 physical and functional interactions. Sci Rep, 2016.
Ayling LJ, Briddon SJ, Hall, ML, Hammon, GRV, Vac L,
Pacheco J
, Hill SJ and Cooper DMF. Adenylyl cyclase AC8 directly controls its micro-environment by recruiting the actin cytoskeleton in a cholesterol-rich milieu. J Cell Sci, 2012.
Willoughby D, Everett KL, Halls ML,
Pacheco J
, Skroblin P, Vaca L, Klussmann E and Cooper DMF. Direct binding between Orai1 and AC8 mediates dynamic interplay between Ca2+and cAMP signaling. Sci Signal, 2012.